Membrane Anchored TNFα Cell (Adherent)
CBP74152
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| I. Background | |
| 腫瘤壞死因子-α（TNF-α）是一種分子量為 26 kDa 的跨膜蛋白，其前肽在 TNF-α-轉(zhuǎn)換 酶（TACE）裂解時(shí)以可溶性 17 kDa 分子的形式分泌。雖然活化的巨噬細(xì)胞是 TNF-α的主 要來(lái)源，但它也可以由多種其他細(xì)胞產(chǎn)生，如成纖維細(xì)胞、星形膠質(zhì)細(xì)胞、平滑肌細(xì)胞、 角化細(xì)胞和多種腫瘤細(xì)胞，包括 B 細(xì)胞淋巴瘤、乳腺癌和結(jié)腸癌。 TNF-α作為腫瘤促進(jìn)因子，參與腫瘤發(fā)生的所有步驟，包括腫瘤的轉(zhuǎn)化、增殖、血管 生成、侵襲和轉(zhuǎn)移。TNF α信號(hào)傳導(dǎo)也是免疫系統(tǒng)的重要組成部分，可抑制腫瘤發(fā)生，阻止病毒復(fù)制，是一種內(nèi)源性熱原，可誘導(dǎo)發(fā)燒和細(xì)胞凋亡。 |
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| II. Description | |
| Membrane Anchored TNFα Cell(Adherent)，穩(wěn)定表達(dá)人 TNFα。 | |
| III. Introduction | |
| Expressed gene: | TNFα |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 1. Recombinant Membrane Anchored TNFα Cell (Adherent) constitutively expressing TNFα.
Figure 2. Dose Response of TNFα Abs in ADCC Bioassay Effector Cell V variant (High Affinity)-Fcγ-NFAT-Jurkat (C36) with Membrane Anchored TNFα Cell (Adherent, C12).
Figure 3. Blocking of TNFα induced TNFR2( TNFR1 KO) Effector Reporter Cell(C64) Activity by TNFα Neutralization Abs with Membrane Anchored TNFα Cell(Adherent, C12).
Figure 4. Blocking of TNFα induced TNFR2(TNFR1 KO) Effector Reporter Cell( C64) Activity by TNFR2 Blocking Ab with Membrane Anchored TNFα Cell ( Adherent, C12).
