CD200R Effector Reporter cell
CBP74179
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產(chǎn)品描述
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| I. Background | |
| CD200作為CD200R的同源配體，也是一種IgSF蛋白，在小鼠和人類中，CD200R的表達(dá)模式相似，均在巨噬細(xì)胞、中性粒細(xì)胞和肥大細(xì)胞中高表達(dá)。與大多數(shù)IgSF受體不同，CD200R缺乏ITIM結(jié)構(gòu)域，然而，它的67個(gè)氨基酸組成的細(xì)胞質(zhì)尾部含有三個(gè)酪氨酸殘基，第三個(gè)酪氨酸殘留位于NPXY基序內(nèi)，該基序在CD200R結(jié)合配體后會被磷酸化，這導(dǎo)致其并不直接招募SHP1,SHP2等蛋白質(zhì)酪氨酸磷酸酶或磷酸化后的肌醇磷酸酶（SHIP），而是招募酪氨酸激酶下游銜接蛋白Dok-2和1，然后再與RasGAP和SHIP結(jié)合。 | |
| II. Introduction | |
| Expressed gene: | CD200R |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+10ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1. Recombinant CD200R Effector Reporter cell constitutively expressing CD200R. |
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Figure 2. Dose Response of CD200R Blocking Ab in CD200R Effector Reporter Cells (C34) With CD200 Target cells (C18). |
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