PDGFRA D842V-V658A/BaF3
CBP73333
| I. Introduction | |
|
Cell Line Name： |
PDGFRA D842V-V658A/BaF3 |
|
Host Cell: |
Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
|
Complete Culture Medium: |
RPMI-1640+10%FBS |
|
Mycoplasma Status: |
Negative |
| II. Background | |
|
PDGFRA D842V lies within the protein kinase domain of the Pdgfra protein (UniProt.org). D842V results in ligand-independent phosphorylation of Pdgfra, activation of downstream signaling, and is transforming in culture (PMID: 22745105, PMID: 23752188, PMID: 29533785, PMID: 30253204) and confers resistance to PDGFRA inhibitors (PMID: 30389923, PMID: 18955458). |
|
| III. Representative Data | |
|
1.WB of PDGFRA D842V-V658A/BaF3 Figure 2. PDGFRA D842V-V658A/BaF3 V658A
Figure 3. PDGFRA D842V-V658A/BaF3 D842V |
|
|
3. Anti-proliferation assay |
|
|
Figure 4. CTG Proliferation Assay of BaF3 PDGFRA D842V/V658A Cells (C1). |
|
