DLL4/Notch1 Effector Reporter Cell
CBP74305
| I. Background | |
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DLL4（Delta-like ligand 4） 主要表達于血管內(nèi)皮細胞，通過 “尖端細胞-莖細胞”分 化機制調(diào)控血管分支：DLL4 激活鄰近細胞的 Notch1 信號，抑制其分化為尖端細胞，從而 限制血管過度生長，維持血管網(wǎng)絡(luò)的有序性。在腫瘤微環(huán)境中，DLL4 在異常血管中高表 達，誘導血管結(jié)構(gòu)紊亂（滲漏、低灌注），促進缺氧和化療耐藥。此外，DLL4 參與神經(jīng) 發(fā)育及 T 細胞分化，其基因突變可導致 Adams-Oliver 綜合征等先天性疾病。 |
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| II. Description | |
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DLL4/Notch1 Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) DLL4/Notch1 的信號轉(zhuǎn)導過程，原理見下圖所示。
Figure 1. DLL4/Notch1 Effector Reporter Cell 細胞模型原理圖
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| III. Introduction | |
| Expressed gene: | DLL4/Notch1 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10% FBS+200 μg/ml Hygromycin B+2 μg/ml Puromycin+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant DLL4/Notch1 Effector Reporter Cell stably expressing Notch1.  Figure 3. Blocking of DLL4 induced DLL4/Notch1 Effector Reporter Cell Activity by DLL4 Neutralizing Antibody with DLL4 Target Cell(C7). |
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