BCR-ABL1 [Q252H/T315I] /BaF3
CBP73278
| I. Introduction | |
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Cell Line Name： |
BCR-ABL1 [Q252H/T315I] /BaF3 |
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Host Cell: |
Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
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Application: |
Anti-proliferation assay and PD assay |
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Freeze Medium: |
90% FBS+10% DMSO |
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Complete Culture Medium: |
RPMI-1640+10%FBS |
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Mycoplasma Status: |
Negative |
| II. Background | |
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ABL1, ABL proto-oncogene 1, non-receptor tyrosine kinase, plays a role in regulation of cell growth through ERK5, Rac/JNK, and Stat1/3 pathways (PMID: 10373409). Fusion proteins of BCR-ABL1 resulting in constitutive kinase activity have been reported in various cancers (PMID: 18851712, PMID: 30125955) and a number of Abl1 mutations in the context of BCR-ABL1 have been demonstrated to confer drug resistance (PMID: 24456693). |
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| III. Representative Data | |
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1.WB of BCR-ABL1 [Q252H/T315I] /BaF3
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2. Sanger of BCR-ABL1 [Q252H/T315I] /BaF3
Figure 2. BCR-ABL1 [Q252H/T315I] /BaF3 Fusion
Figure 3. BCR-ABL1 [Q252H/T315I] /BaF3 Q252H Mutation
Figure 4. BCR-ABL1 [Q252H/T315I] /BaF3 T315I Mutation
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3. Anti-proliferation assay
Figure 5 .CTG Proliferation Assay of BaF3 BCR-ABL1 T315I Q252H Cells (C1). |
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