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NCI-N87(人胃癌細胞)

CBP60491

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I. General information 
Synonyms: NCI-N87
Species: Homo sapiens, human
Tissue: stomach; derived from metastatic site: liver
Disease: gastric carcinoma
Gender: male
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 47 hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 8,12
D13S317: 8,11
D16S539: 9,13
D5S818: 12,13
D7S820: 10,11
THO1: 9
TPOX: 9,11
vWA: 15,16
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Culture Medium:

RPMI1640+10%FBS

NCI-N87完全培養(yǎng)基,# CBP60491M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: acetylcholine, muscarinic, expressed ,Ref
Oncogene: myc +; erb B2 +
Comments: NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were found to express receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B 2 RNA that were comparable to other cell lines.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%.
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